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毕赤

网络Pichia pastoris

毕赤

毕赤

Pichia pastoris

...出发菌株,克隆植酸酶phyA基因,并将其连接构建到毕赤(Pichia pastoris)酵母分泌型表达载体pPICZαA中,利用电击转化的方法 …

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Pichia pastoris is one of the most used expression systems for the high level expression of heterologous proteins .

巴斯德毕赤酵母是目前应用最广泛的外源蛋白表达系统。

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Methods The annexin V fused with a metal chelating binding site was obtained from pichia pastoris culture and methanol induction expression.

方法带有金属螯合位点的膜联蛋白 V ，通过毕赤酵母的培养和甲醇诱导表达获得；

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The invention relates to a Paris polyphylla var. yunnanensis endogenetic Pichia pastor is used to produce volatile oil .

本发明涉及一种产挥发油的滇重楼内生毕赤酵母。

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Results revealed that P . stipitis could utilize levoglucosan at a low efficiency compared to glucose , and could not ferment it to ethanol.

结果表明，与对葡糖糖的利用相比，树干毕赤酵母菌利用内醚糖的效率较低，且不能发酵产乙醇。

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In our lab , genetically engineered yeast Pichia Pastoris which boring human insulin gene was used to express heterologous protein .

本实验室将人胰岛素原基因整合到甲醇营养型酵母巴斯德毕赤酵母中，构成基因工程菌，诱导表达重组人胰岛素原。

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Displayed on the pichia surface , CALB not only achieved self-immobilization, also has it's heat resistance and catalytic activity improved .

将其展示于毕赤酵母表面展示，不但实现了自固定化，耐热性和催化活性也有改善。

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Objective : To express ancrod, a snake venom thrombin-like enzyme, in methylotrophic yeast Pichia pastoris.

目的：利用毕赤酵母表达具有生物学活性的蛇毒类凝血酶安克洛。

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Experiment 1: High cell density fermentation of Pichia pastoris included three phases : batch , glycerol fed-batch and induction phase .

试验 1 ：采用毕赤酵母高密度发酵，分分批、补料和诱导三个阶段进行。

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Conclusion The annexin V expressed recombinantly in pichia pastoris show higher affinity to PS exposed erythrocytes .

结论毕赤酵母系统重组表达的膜联蛋白 V 具有较高的结合外露 PS 红细胞的能力。

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Chen GQ, Wang HT , Li ZG and Wu Q. Expression and purification of human Tyrosinase in Pichia pasteuris .

人酪氨酸酶在毕赤巴斯德酵母中的表达和纯化。

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Prime research of phytase 's characteristics from gene-engineering recombined yeasts

重组毕赤酵母产植酸酶的性质初步研究

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Construction of two new multi-copy Pichia expression vectors

两个新型多拷贝毕赤酵母表达载体的构建

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Advance of pichia yeast expression system applied in animal infectious disease agent gene

毕赤酵母表达动物传染病病原基因的研究进展

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