大家在查： Markey Ford，Corker 亚历山大麦昆黄国伟白种人温德尔·史蒂文森 RickMayer Clippard 折戟沉沙 SergioMarchionn

pastoris

pastoris

pastoris

pastoris

1

Pichia pastoris is one of the most used expression systems for the high level expression of heterologous proteins .

巴斯德毕赤酵母是目前应用最广泛的外源蛋白表达系统。

2

Methods The annexin V fused with a metal chelating binding site was obtained from pichia pastoris culture and methanol induction expression.

方法带有金属螯合位点的膜联蛋白 V ，通过毕赤酵母的培养和甲醇诱导表达获得；

5

In our lab , genetically engineered yeast Pichia Pastoris which boring human insulin gene was used to express heterologous protein .

本实验室将人胰岛素原基因整合到甲醇营养型酵母巴斯德毕赤酵母中，构成基因工程菌，诱导表达重组人胰岛素原。

7

Objective : To express ancrod, a snake venom thrombin-like enzyme, in methylotrophic yeast Pichia pastoris.

目的：利用毕赤酵母表达具有生物学活性的蛇毒类凝血酶安克洛。

8

Experiment 1: High cell density fermentation of Pichia pastoris included three phases : batch , glycerol fed-batch and induction phase .

试验 1 ：采用毕赤酵母高密度发酵，分分批、补料和诱导三个阶段进行。

9

Conclusion The annexin V expressed recombinantly in pichia pastoris show higher affinity to PS exposed erythrocytes .

结论毕赤酵母系统重组表达的膜联蛋白 V 具有较高的结合外露 PS 红细胞的能力。

10

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